Antibiotics
An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting bacterial infections, and antibiotic medications are widely used in the treatment and prevention of such infections. They may either kill or inhibit the growth of bacteria.
Blasticidin S HCl (10 mg/mL)
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
Specifications
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Concentration –
10 mg/mL
Culture Type –
Mammalian Cell Culture, Insect Cell Culture
Form –
Liquid
Product Type –
Antibiotic
Shelf Life –
9 Months
Sterility –
Sterile-filtered
With Additives
–
HEPES
Contents & Storage
Storage conditions:
-5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture
Blasticidin S HCl, powder
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
Specifications
Color –
White, off-white
Cell Type –
Eukaryotic Cells, Prokaryotic Cells
Concentration –
50 to 100 μg/mL
Culture Type –
Mammalian Cell Culture, Insect Cell Culture
Form –
Powder
Product Type –
Antibiotic
Shelf Life –
48 Months
Sterility –
Sterile
With Additives–
HEPES
For Use With (Application) –
Bacterial Selection
Contents & Storage
Storage conditions:
-5°C to -30°C
Shipping conditions:
room temperature
Shelf life: 48 months from date of manufacture
Puromycin Dihydrochloride
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene. Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.
Specifications
Concentration – 10 mg/mL
Form – Liquid
Product Type – Antibiotic
Shelf Life – 12 Months
Sterility – Sterile-filtered
With Additives – HEPES
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Quantity – 10 vials
Shipping Condition – Dry Ice
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture