Chromatography is the technique for the separation, purification, and testing of compounds. Chromatography is a separation method where the analyte is combined within a liquid or gaseous mobile phase., which is pumped through a stationary phase. Usually one phase is hydrophilic and the other is lipophilic. The components of the analyte interact differently with these two phases. Depending on their polarity they spend more or less time interacting with the stationary phase and are thus retarded to a greater or lesser extent. This leads to the separation of the different components present in the sample. Each sample component elutes from the stationary phase at a specific time called as retention time. As the components pass through the detector their signal is recorded and plotted in the form of a chromatogram.