Amphotericin B

Amphotericin B is the generic version of Fungizone. ‘Fungizone’ is a trademark of E.R. Squibb & Sons, LLC. Amphotericin B is an antifungal, produced by Streptomyces nodosus. It prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. It actively binds to sterols and leads to the formation of pores. It is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco Amphotericin B contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/mL.

Specifications

Concentration – 0.25 to 2.5 μg/mL
Form – Liquid
Product Type – Antifungal
Shelf Life – 12 Months
Sterility – Sterile
For Use With (Application) – Prevention of Cell Culture Contamination
Quantity – 50 mL
Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Frozen on dry ice
Shelf life: 12 months from date of manufacture

Antibiotic-Antimycotic (100X)

Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast. Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.

Specifications

Concentration – 100 X
Culture Type – Mammalian Cell Culture
Form – Liquid
Product Type – Antibiotic-Antimycotic
Shelf Life – 12 Months
Sterility – Sterile-filtered
Quantity – 100 mL
Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

Gentamicin (10 mg/mL)

Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used for the prevention of bacterial contamination on cell cultures. The recommended working concentration ranges from 0.5 to 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Specifications

Concentration – 10 mg/mL
Culture Type – Mammalian Cell Culture
Form – Liquid
Product Type – Antibiotic
Shelf Life – 24 Months
Sterility – Sterile-filtered
For Use With (Application) – Prevention of Cell Culture Contamination
Quantity – 10 mL
Shipping Condition – Room Temperature

Contents & Storage

Storage conditions: 15°C to 30°C
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture

Gentamicin (10 mg/mL)

Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used for the prevention of bacterial contamination on cell cultures. The recommended working concentration ranges from 0.5 to 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Specifications

Type – Gentamicin
Physical Form – Liquid
Sterile Filtered – Yes
Concentration – 10 mg⁄ml, 10 mg⁄mL
Quantity – 20 x 100 mL Validated Application Prevention of Cell Culture Contamination
Green Features – Sustainable packaging

Contents & Storage

Storage conditions: 15-30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Gentamicin (50 mg/mL)

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 µg /mL.

Specifications

Type – Gentamicin
Physical Form – Liquid
Sterile Filtered – Yes
Concentration – 50 mg/mL
Quantity – 10 x 10 mL
Validated Application – Prevention of Cell Culture Contamination
Shipping Condition – Room Temperature

Contents & Storage

Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

Gentamicin (50 mg/mL)

Gentamicin (gentamicin sulfate) is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and promotion of cell death in susceptible bacteria.

Specifications

Type – Gentamicin
Physical Form – Liquid
Concentration – 50 mg⁄mL
Quantity – 20 x 100 mL
Validated Application – Prevention of Cell Culture Contamination
Green Features – Sustainable packaging

Geneticin Selective Antibiotic (G418 Sulfate), Powder

Geneticin Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin™ Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 – 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10 – 50 mg/ml in water. We offer a variety of cell culture antibiotics for your convenience

Specifications

Cell Type – Eukaryotic Cells, Prokaryotic Cells
Concentration – 10 to 50 μg/mL
Form – Powder
Product Type – Antibiotic
Shelf Life – 24 Months
Sterility – Sterile
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Product Line – Geneticin
Quantity – 1 g
Shipping Condition – Room Temperature

Contents & Storage

Storage conditions: 15 to 30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Hygromycin B (50 mg/mL)

Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Gibco™ Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution.

Specifications

Concentration – 50 mg/mL
Culture Type – Mammalian Cell Culture, Insect Cell Culture
Form – Liquid
Product Type – Antibiotic
Sterility – Sterile
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Shipping Condition – Room Temperature

Contents & Storage

Storage conditions: 2 to 8°C
Shipping conditions: Ambient

Penicillin-Streptomycin (5,000 U/mL)

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria. This solution contains 5000 units/mL of penicillin and 5000 µg/mL of streptomycin.

Specifications

Concentration – 100 X
Culture Type – Mammalian Cell Culture, Insect Cell Culture
Form – Liquid Product Type – Antibiotic Shelf Life – 12 Months Sterility – Sterile-filtered For Use With (Application) – Prevention of Cell Culture Contamination Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

Penicillin-Streptomycin (10,000 U/mL)

This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria.

Specifications

Concentration – 100 X
Form – Liquid
Product Type – Antibiotic
Shelf Life – 12 Months
Sterility – Sterile-filtered
For Use With (Application) – Prevention of Cell Culture Contamination
Quantity – 100 mL
Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

Penicillin-Streptomycin-Glutamine (100X)

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . It acts by binding to the 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria. This solution contains 10,000 units of penicillin, 10,000 µg of streptomycin, and 29.2 mg/ml of L-glutamine in a 10 mM citrate buffer (for pH stability).

Specifications

Concentration – 100 X, 50 mg/mL
Form – Liquid
Product Type – Antibiotic-L-Glutamine
Shelf Life – 12 Months
Sterility – Sterile-filtered
With Additives – Glutamine
For Use With (Application) – Prevention of Cell Culture Contamination
Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture

The antibiotics Penicillin, Streptomycin, and Neomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus and Neomycin from Streptomyces fradiae. Streptomycin and Neomycin act by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. We offer a variety of antibiotics and antimycotics for cell culture applications.

Specifications

Concentration – 100 X
Culture type – Mammalian Cell Culture
Form – Liquid
Product Type – Antibiotic
Sterility – Sterile-filtered
For Use With (Application) – Prevention of Cell Culture Contamination
Shipping Condition – Dry Ice

Contents & Storage

Store in freezer (-5 to -30°C).

Puromycin Dihydrochloride

Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene. Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.

Specifications

Concentration – 10 mg/mL
Form – Liquid
Product Type – Antibiotic
Shelf Life – 12 Months
Sterility – Sterile-filtered
With Additives – HEPES
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Quantity – 10 vials
Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture

Zeocin Selection Reagent

Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. Zeocin Selection Antibiotic acts by binding to and cleaving DNA, leading to cell death. Zeocin Selection Antibiotic is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. It is used at a working concentration ranging between 50–400 µg/mL depending on the cell type. This product is supplied at 100 mg/mL in sterile water in one 50 mL bottle.

Specifications

Concentration – 100 mg/mL
Culture Type – Mammalian Cell Culture, Insect Cell Culture
Form – Liquid
Product Type – Antibiotic
Shelf Life – 12 Months
Sterility – Sterile-filtered
For Use With (Application) – Eukaryotic Selection/Stable Cell Line Generation, Bacterial Selection
Product Line – Zeocin
Quantity – 8 x 1.25 mL

Contents & Storage

Storage conditions: -10 to -30°C
Shipping conditions: Ice