DTT, IPTG, X-gal, X-gluc, TMB dihydrochloride, MB Free Base, X Gal, Ampicillin, Bialaphos, Carbenicillin, Cefotaxime, Chloramphenicol, Coelenterazine, D-Luciferin Potassium Salt, D-Luciferin Sodium Salt, G418 Sulphate, Hygromycin B, Kanamycin, Luminol, MUG, NBT, Phosphinothricin, Rifampicin, Spectinomycin, Streptinomycin, Ticarcillin/Clavulanate, Vancomycin, Antibiotics Solution, Beads & Purification, plant growth regulator, Media component, Protein Tools.
X-Gluc is a chromogenic indicator for beta-glucuronidase activity. X-Gluc can also be used as detecting agent for beta-glucuronidase activity in reporter gene assays or for identification of positive transformants, which is of particular value in plant science (GUS staining). Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gluc. The reaction proceeds only in the presence of oxygen, i.e., in aerobic cultures. Well detectable colored bacterial colonies or liquid cultures are obtained within 16-48 h. In the presence of purified beta-galactosidase, color develops within minutes to few hours.