Antibiotics

An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting bacterial infections, and antibiotic medications are widely used in the treatment and prevention of such infections. They may either kill or inhibit the growth of bacteria.

Amphotericin B

Amphotericin B is the generic version of Fungizone. ‘Fungizone’ is a trademark of E.R. Squibb & Sons, LLC. Amphotericin B is an antifungal, produced by Streptomyces nodosus. It prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. It actively binds to sterols and leads to the formation of pores. It is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco Amphotericin B contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/mL.

Specifications

Concentration – 0.25 to 2.5 μg/mL
Form – Liquid
Product Type – Antifungal
Shelf Life – 12 Months
Sterility – Sterile
For Use With (Application) – Prevention of Cell Culture Contamination
Quantity – 50 mL
Shipping Condition – Dry Ice

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Frozen on dry ice
Shelf life: 12 months from date of manufacture

Hygromycin B (50 mg/mL)

Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution.

Specifications

Concentration – 50 mg/mL
Culture Type – Mammalian Cell Culture, Insect Cell Culture
Form – Liquid
Product Type – Antibiotic
Sterility – Sterile
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Shipping Condition – Room Temperature

Contents & Storage

Storage conditions: 2 to 8°C
Shipping conditions: Ambient

Blasticidin S HCl, powder

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

Specifications

Color – White, off-white
Cell Type – Eukaryotic Cells, Prokaryotic Cells
Concentration – 50 to 100 μg/mL
Culture Type – Mammalian Cell Culture, Insect Cell Culture
Form – Powder
Product Type – Antibiotic
Shelf Life – 48 Months
Sterility – Sterile
With Additives– HEPES
For Use With (Application) – Bacterial Selection

Contents & Storage

Storage conditions: -5°C to -30°C
Shipping conditions: room temperature
Shelf life: 48 months from date of manufacture

Geneticin Selective Antibiotic (G418 Sulfate), Powder

Geneticin Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin™ Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Geneticin™ Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 – 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10 – 50 mg/ml in water. We offer a variety of cell culture antibiotics for your convenience

Specifications

Cell Type – Eukaryotic Cells, Prokaryotic Cells
Concentration – 10 to 50 μg/mL
Form – Powder
Product Type – Antibiotic
Shelf Life – 24 Months
Sterility – Sterile
For Use With (Application) – Eukaryotic Selection⁄Stable Cell Line Generation
Product Line – Geneticin
Quantity – 25 g
Shipping Condition – Room Temperature

Contents & Storage

Storage conditions: 15 to 30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture