Transcription Factors

Antibodies

Antibodies are proteins that protect you when an unwanted substance enters your body. Produced by your immune system, antibodies bind to these unwanted substances in order to eliminate them from your system. Another word for antibody is immunoglobulin.

Transcription Factors

NF-kB

Anti-NF-kB p65 antibody
Description

Mouse monoclonal [27F9.G4] to NF-kB p65

Specifications
Concentration –

50 µg at 1 mg/ml

Isotype –

IgG2a

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

NF-kB Signaling Pathway Antibody Sampler Panel
Description

NF-kB Signaling Pathway Antibody Panel, is a collection of 1 anti-rabbit (HRP) secondary antibody and 5 recombinant rabbit monoclonal antibodies against IKB alpha (p S36), IKB alpha, NFkB p105, NF-kB p65 (p S536), and NF-kB p65 provided in 10 µl trial sizes.

Specifications
Storage –

Store at -20°C. Please refer to protocols.

APC Anti-NF-kB p65 antibody
Description

APC Rabbit monoclonal [NFkBp65S529-H3] to NF-kB p65 (phospho S529)

Specifications
Conjugate –

APC. Ex: 645nm, Em: 660nm

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C. Store In the Dark.

Biotin Anti-NF-kB p65 antibody
Description

Biotin Rabbit monoclonal [E379] to NF-kB p65

Specifications
Concentration –

100 µl at 0.5 mg/ml

Conjugate –

Biotin

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

FITC Anti-NF-kB p65 antibody
Description

FITC Rabbit monoclonal [NFkBp65S529-H3] to NF-kB p65 (phospho S529)

Specifications
Conjugate –

FITC. Ex: 493nm, Em: 528nm

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C. Store In the Dark.

Monoclonal Anti-NF-κB antibody produced in mouse
Description

Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors. Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.

Specifications
Conjugate –

unconjugated

Isotype –

IgG1

Storage –

-20°C.

Anti-NF-κB p100 antibody produced in rabbit
Description

Anti-NF-κB p100 Antibody detects endogenous levels of total NF-κB p100 protein.

Specifications
Concentration –

~1 mg/mLL

Conjugate –

unconjugated

Isotype –

IgG

Form –

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage –

-20°C.

Anti-NF-κB p105 antibody produced in rabbit
Description

Anti-NF-κB p105 Antibody detects endogenous levels of total NF-κB p105 protein. The gene NFκB1 (nuclear factor κ B subunit 1) is mapped to human chromosome 4q24. It codes for NF-κB p105/p50 isoforms.

Specifications
Concentration –

~1 mg/mL

Conjugate –

unconjugated

Isotype –

IgG

Form –

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage –

-20°C.

C-Myc

Anti-c-Myc−Biotin antibody, Mouse monoclonal
Description

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Specifications
Concentration –

0.8 mg/mL

conjugate –

biotin conjugate

Isotype –

IgG

Form –

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Storage –

−20°C

Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal
Description

Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62c-myc is associated mainly with cell nuclei.

Specifications
conjugate –

alkaline phosphatase conjugate

Isotype –

IgG1

Form –

Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Storage –

2-8°C

Rabbit anti-c-myc Antibody
Description

 Rabbit anti-c-myc Antibody

Specifications
Isotype –

IgG

Form –

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide

Storage –

2-8°C.

Anti-acetyl-c-Myc (Lys148) Antibody
Description

c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell growth, differentiation, and proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling. c-Myc is itself acetylated on lysine 323 by GCN5, and on lysine 148 and 157 by p300/CBP. The significance of c-Myc acetylation is still under investigation; however, previous studies have suggested that HAT-dependent acetylation of c-Myc may regulate its turnover and stability. c-Myc may play a role in various cancers such as Burkitt’s lymphoma.

Specifications</h6 >
Isotype –

IgG

Form –

Affinity purified Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage –

Stable for 1 year at 2-8°C from date of receipt.

Anti-c-Myc-Peroxidase antibody, Mouse monoclonal
Description

Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide of the human p62c-myc protein, conjugated to KLH. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain. The human c-Myc proto-oncogene is the cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses.

Specifications
Concentration –

~2 mg/mL

Conjugate –

peroxidase conjugate

Isotype –

IgG1

Form –

lyophilized powder

Storage –

−20°C

Anti-c-Myc antibody
Description

Rabbit monoclonal [Y69] to c-Myc – ChIP Grade

Specifications
Concentration –

Batch dependent within range: 100 µl at 0.216 – 0.223 mg/ml

Isotype –

IgG

Form –

Liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

HRP Anti-c-Myc antibody
Description

HRP Rabbit polyclonal to c-Myc

Specifications
Concentration –

100 µg at 1 mg/ml

Con jugate –

HRP

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C.

FITC Anti-c-Myc antibody
Description

FITC Rabbit monoclonal [Y69] to c-Myc

Specifications
Concentration –

100 µl at 0.5 mg/ml

Conjugate –

FITC. Ex: 493nm, Em: 528nm

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

Human c-Myc Antibody
Description

ELISA set

Specifications
Concentration –

~1 mg/ml

Isotype –

IgG

Form –

liquid

Storage –

Store at +4°C. Please refer to protocols.

PE Anti-c-Myc antibody
Description

PE Rabbit monoclonal [EPR17923] to c-Myc (phospho T58)

Specifications
Concentration –

100 µl at 0.5 mg/ml

Conjugate –

PE. Ex: 488nm, Em: 575nm

Isotype –

IgG

Form –

liquid

Storage –

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.